Solubility-Based Phase Partitioning of Mouse Liver Microsomes Using Triton X-114
نویسندگان
چکیده
منابع مشابه
Removal of endotoxin from protein solutions by phase separation using Triton X-114.
Endotoxin contamination of protein solutions was reduced by a phase separation technique using the detergent, Triton X-114. Protein solutions containing endotoxin were treated with Triton X-114 on ice. The solution was then warmed to 37 degrees C, whereupon two phases formed. The Triton X-114 phase, containing the endotoxin, was precipitated by centrifugation. The first cycle of phase separatio...
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ژورنال
عنوان ژورنال: Journal of Proteomics & Bioinformatics
سال: 2008
ISSN: 0974-276X
DOI: 10.4172/jpb.s1000090